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Microcystin-LR biodegradation by

Chengbin XIAO, Hai YAN, Junfeng WANG, Wei WEI, Jun NING, Gang PAN

《环境科学与工程前沿(英文)》 2011年 第5卷 第4期   页码 526-532 doi: 10.1007/s11783-010-0261-7

摘要: A promising bacterial strain for biodegrading microcystin-LR (MC-LR) as the sole carbon and nitrogen source was successfully isolated from Lake Dianchi, China. The strain was identified as sp. USTB-05, which was the first isolated MCs-biodegrading sp. in China. The average biodegradation rate of MC-LR by sp. USTB-05 was 28.8 mg·L per day, which was apparently higher than those of other bacteria reported so far. The optimal temperature and pH for both strain USTB-05 growth and MC-LR biodegradation were 30°C and 7.0, respectively. The release of MC-LR from the cyanobacterial cells collected from Lake Guishui and the biodegradation of MC-LR by both strain and cell-free extract (CE) were investigated. The results indicated that MC-LR with the initial concentration of 4.0 mg·L in water was biodegraded by sp. USTB-05 within 4 d, while MC-LR with the initial concentration of 28.8 mg·L could be completely removed in 3 h by CE of sp. USTB-05 containing 350 mg·L protein. During enzymatic biodegradation of MC-LR, two intermediate metabolites and a dead-end product were observed on an HPLC chromatogram. Moreover, the similar scanning profiles of MC-LR and its metabolic products indicate that the Adda side-chain of MC-LR was kept intact in all products.

关键词: microcystin-LR(MC-LR)     biodegradation     Sphingopyxis sp     USTB-05     cell-free extract    

Oxidation and biotoxicity assessment of microcystin-LR using different AOPs based on UV, O

Siyi Lu, Naiyu Wang, Can Wang

《环境科学与工程前沿(英文)》 2018年 第12卷 第3期 doi: 10.1007/s11783-018-1030-2

摘要: Microcystin-LR attracts attention due to its high toxicity, high concentration and high frequency. The removal characteristics of UV/H O and O /H O advanced oxidation processes and their individual process for MC-LR were investigated and compared in this study. Both the removal efficiencies and rates of MC-LR as well as the biotoxicity of degradation products was analyzed. Results showed that the UV/H O process and O /H O were effective methods to remove MC-LR from water, and they two performed better than UV-, O -, H O -alone processes under the same conditions. The effects of UV intensity, H O concentration and O concentration on the removal performance were explored. The synergistic effects between UV and H O , O and H O were observed. UV dosage of 1800 mJ·cm was required to remove 90% of 100 g·L MC-LR, which amount significantly decreased to 500 mJ·cm when 1.7 mg·L H O was added. 0.25 mg·L O , or 0.125 mg·L O with 1.7 mg·L H O was needed to reach 90% removal efficiency. Furthermore, the biotoxicity results about these UV/H O , O /H O and O -alone processes all present rising trends with oxidation degree of MC-LR. Biotoxicity of solution, equivalent to 0.01 mg·L Zn , raised to 0.05 mg·L Zn after UV/H O or O /H O reaction. This phenomenon may be attributed to the aldehydes and ketones with small molecular weight generated during reaction. Advice about the selection of MC-LR removal methods in real cases was provided.

关键词: Microcystin-LR     Advanced oxidation processes (AOPs)     Biotoxicity     Synergistic effects    

Comparison of different algicides on growth of

Naiyu Wang, Kai Wang, Can Wang

《环境科学与工程前沿(英文)》 2017年 第11卷 第6期 doi: 10.1007/s11783-017-0940-8

摘要: Eutrophication with a large number of commonly occurs worldwide, thereby threatening the aquatic ecosystem and human health. In this study, four kinds of algicides were tested to explore their influence on cell density and chlorophyll-a of . Results showed that aluminum silicate agent, which inhibited more than 90% cell growth compared with the control group, demonstrated the strongest inhibition effect immediately on growth. Furthermore, the production and release of microcystin (MC)-LR were investigated. Aluminum silicate, CuSO , and Emma-11 were more effective than pyrogallic acid in disrupting the cells of , thereby increasing the extracellular MC-LR concentration. Aluminum silicate caused the highest extracellular MC-LR concentration of more than 45 mg·L . Biotoxicity was also detected to evaluate the environmental risks of MC-LR release, which were related to the usages of different algicides. Extracellular MC-LR concentration mostly increased when the biotoxicity of algae solution increased. The experiments were also designed to reveal the effects of physical conditions in riverways, such as natural sunlight, aeration and benthal sludge, on MC-LR degradation. These findings indicated that UV rays in sunlight, which can achieve a MC-LR removal efficiency of more than 15%, played an important role in MC-LR degradation. Among all the physical pathways of MC-LR removal, benthal sludge adsorption presented the optimal efficiency at 20%.

关键词: Microcystis aeruginosa     MC-LR release     Algicides     MC-LR degradation     Biotoxicity    

Microcystin-LR detection based on indirect competitive enzyme-linked immunosorbent assay

SHENG Jianwu, HE Miao, YU Shaoqing, SHI Hanchang, QIAN Yi

《环境科学与工程前沿(英文)》 2007年 第1卷 第3期   页码 329-333 doi: 10.1007/s11783-007-0056-7

摘要: Microcystins (MCs) are a group of closely related toxic cyclic heptapeptides produced by common cyanobacteria, which cause lots of accidents and threatens human health. In this paper, an indirect competitive enzyme-linked immunosorbent assay (ic-ELISA) was established and used to detect microcystin-LR (MC-LR) in drinking and surface waters. The concentration of coating antigen was 5 ?g/mL, the dilution of monoclonal antibody MC10E7 was 1:3 000, the dilution of enzyme tracer (goat anti-mouse IgG-peroxidase) was 1:3 000, the standard concentration of MC-LR ranged from 0.001 μg/L to 30 μg/L, and o-phenylenediamine was used as substrate. The assay showed high relativity with high performance liquid chromatography (HPLC) with a correlation coefficient of more than 99%. The relative standard deviation was less than 10%, the detection limit was achieved down to 0.01 μg/L and up to 5.1 μg/L. The quantitative detection range was from 0.03 μg/L to 3 μg/L, and the antibody had high specificity for [4-arginine] microcystins. It performed well in spite of the influence of the real samples.

关键词: o-phenylenediamine     4-arginine     ic-ELISA     substrate     chromatography    

工程网络计划的LR型模糊系数线性规划方法

高朋,冯俊文

《中国工程科学》 2009年 第11卷 第2期   页码 70-74

摘要: 文章提出一种具有LR型模糊数的线性规划模型,解决了工程网络计划的时间参数 估计和关键路径识别问题,并通过引入λ截集来充分描述决策者在不同情形下对工序工期估计的可信程度。

关键词: 工程网络计划     模糊线性规划     工序    

激光去除与连接技术发展研究

陈俐,巩水利,何恩光

《中国工程科学》 2020年 第22卷 第3期   页码 78-84 doi: 10.15302/J-SSCAE-2020.03.012

摘要:

随着激光技术的发展,激光去除与连接技术深刻影响着制造业技术的发展,并以柔性、高效、高质的综合优势成为不可或缺的先进制造技术。在先进制造智能化发展的时代,激光技术、数字信息技术等的融合必将推动激光去除与连接技术的应用发展与创新。本文选取工业应用最广的激光焊接、激光切割、激光打孔、激光打标4 种激光去除与连接技术,概述了激光去除与连接技术的应用现状,分析了其在支撑先进制造发展过程中存在的问题。研究表明,激光去除与连接技术的发展应注重工艺基础和装备研究一体化的研究,解决激光加工单元部件、激光加工工艺、智能集成各环节的关键技术,强化工艺基础研究平台建设,关注技术创新和人才培养的多维度结合,以促进激光去除与连接技术从工艺应用开发到装备市场保障的生态链式的协同发展。

关键词: 激光加工     激光焊接     激光切割     激光打孔     激光打标    

Temperature field simulation of laser homogenizing equipment

Juanjuan WANG, Yunshan WANG, Fudong ZHU

《机械工程前沿(英文)》 2009年 第4卷 第1期   页码 49-52 doi: 10.1007/s11465-009-0005-1

摘要: The laser homogenizing equipment was devised using the ring scanning principle. Its working principle is explained. A laser scanning ring facula is obtained when the laser beam goes through the equipment’s optical system rotating with high-frequency. The scanning ring facula’s mathematic model is established based on the temperature field’s superposing principle. The ring facula’s light intensity distribution and temperature distribution characteristics are achieved by simulating its temperature field. By studying the effect of parameters on the temperature field, the best parameter can be found. Results show that favorable temperature distribution characteristics can be attained by choosing appropriate parameters, and even the thermal effect can be realized by utilizing the circumference power compensating for the heat exchange lost in the horizontal direction. The uniform hardness layer and better process quality can be attained using the ring facula optimized for metal laser heat treatment.

关键词: laser homogenizing technology     laser scanning ring facula     temperature field    

我国激光技术与应用2035 发展战略研究

“我国激光技术与应用2035 发展战略研究”项目综合组

《中国工程科学》 2020年 第22卷 第3期   页码 1-6 doi: 10.15302/J-SSCAE-2020.03.001

摘要:

为促进激光技术及应用的发展,中国工程院于2018 年启动了“我国激光技术与应用2035 发展战略研究”重点咨询项目,本文是项目研究综合报告,文章简要分析了激光技术及在科研与产业中的工具性、引领性和颠覆性作用,分析了我国激光技术在前沿激光、制造加工、信息通信、医疗诊治等领域的科研和应用的发展现状,梳理了多个制约加速发展的问题,提出了我国激光技术及应用2035 年发展目标设想:强化顶层统筹谋划,引领我国激光技术与应用产业快速发展;加强基础研究,聚力突破产业化发展中的重大基础问题、共性技术基础和基础材料、器件;设立应用研发专项,打造多个新激光产业链;创新产业协同机制,完善激光产业创新生态;强化激光基础教育,加大对激光及光学学科人才培养力度等政策措施建议。

关键词: 激光技术     激光制造     激光通信     激光诊疗    

Processing parameter optimization of fiber laser beam welding using an ensemble of metamodels and MOABC

《机械工程前沿(英文)》 2022年 第17卷 第4期 doi: 10.1007/s11465-022-0703-5

摘要: In fiber laser beam welding (LBW), the selection of optimal processing parameters is challenging and plays a key role in improving the bead geometry and welding quality. This study proposes a multi-objective optimization framework by combining an ensemble of metamodels (EMs) with the multi-objective artificial bee colony algorithm (MOABC) to identify the optimal welding parameters. An inverse proportional weighting method that considers the leave-one-out prediction error is presented to construct EM, which incorporates the competitive strengths of three metamodels. EM constructs the correlation between processing parameters (laser power, welding speed, and distance defocus) and bead geometries (bead width, depth of penetration, neck width, and neck depth) with average errors of 10.95%, 7.04%, 7.63%, and 8.62%, respectively. On the basis of EM, MOABC is employed to approximate the Pareto front, and verification experiments show that the relative errors are less than 14.67%. Furthermore, the main effect and the interaction effect of processing parameters on bead geometries are studied. Results demonstrate that the proposed EM-MOABC is effective in guiding actual fiber LBW applications.

关键词: laser beam welding     parameter optimization     metamodel     multi-objective    

Development of lunar regolith composite and structure via laser-assisted sintering

《机械工程前沿(英文)》 doi: 10.1007/s11465-021-0662-2

摘要: Aiming at the exploration and resource utilization activities on the Moon, in situ resource utilization and in situ manufacturing are proposed to minimize the dependence on the ground transportation supplies. In this paper, a laser-assisted additive manufacturing process is developed to fabricate lunar regolith composites with PA12/SiO2 mixing powders. The process parameters and composite material compositions are optimized in an appropriate range through orthogonal experiments to establish the relationship of process–structure–property for lunar regolith composites. The optimal combination of composite material compositions and process parameters are mixing ratio of 50/50 in volume, laser power of 30 W, scanning speed of 3500 mm/s, and scanning hatch space of 0.2 mm. The maximum tensile strength of lunar regolith composites reaches 9.248 MPa, and the maximum depth of surface variation is 120.79 μm, which indicates poor powder fusion and sintering quality. Thereafter, the mechanical properties of laser-sintered lunar regolith composites are implemented to the topology optimization design of complex structures. The effectiveness and the feasibility of this laser-assisted process are potentially developed for future lightweight design and manufacturing of the solar panel installed on the lunar rover.

关键词: in situ manufacturing     laser-assisted powder fusion process     mechanical properties     topological structure design    

激光表面改性技术发展研究

张群莉,王梁,梅雪松,姚建华

《中国工程科学》 2020年 第22卷 第3期   页码 71-77 doi: 10.15302/J-SSCAE-2020.03.010

摘要:

激光表面改性技术利用高能量密度的激光束对零部件局部易损易耗区域进行快速处理,获得材料表面所需性能,进而大幅延长零部件使用寿命,已在航空航天、石油化工、能源、交通、冶金等领域获得应用。本文针对激光冲击强化、激光淬火、激光熔覆3 种典型的激光表面改性技术,梳理了相应技术特点及国内外应用现状,凝练我国相关技术领域面临的差距与问题,总结激光表面改性技术的重点发展方向:专用合金材料制备、多能场激光复合表面改性、面向现场的激光再制造、激光冲击强化控形控性、激光表面改性智能化、激光表面微结构化。从政府引导、国产化创新、产业链完善、质量评价标准体系、人才培养等方面提出发展建议,以期为我国激光表面改性技术发展提供参考借鉴。

关键词: 激光表面改性     冲击强化     淬火     熔覆     发展趋势    

Advances in polishing of internal structures on parts made by laser-based powder bed fusion

《机械工程前沿(英文)》 2023年 第18卷 第1期 doi: 10.1007/s11465-022-0724-0

摘要: The internal structures of metallic products are important in realizing functional applications. Considering the manufacturing of inner structures, laser-based powder bed fusion (L-PBF) is an attractive approach because its layering principle enables the fabrication of parts with customized interior structures. However, the inferior surface quality of L-PBF components hinders its productization progress seriously. In this article, process, basic forms, and applications relevant to L-PBF internal structures are reviewed comprehensively. The causes of poor surface quality and differences in the microstructure and property of the surface features of L-PBF inner structures are presented to provide a perspective of their surface characteristics. Various polishing technologies for L-PBF components with inner structures are presented, whereas their strengths and weaknesses are summarized along with a discussion on the challenges and prospects for improving the interior surface quality of L-PBF parts.

关键词: laser-based powder bed fusion     polishing     internal structures     surface quality     surface features     post process     additive manufacturing    

zone-layered trimming method for ceramic core of aero-engine blade based on an advanced reconfigurable laser

《机械工程前沿(英文)》 2022年 第17卷 第2期 doi: 10.1007/s11465-022-0675-5

摘要: Ceramic structural parts are one of the most widely utilized structural parts in the industry. However, they usually contain defects following the pressing process, such as burrs. Therefore, additional trimming is usually required, despite the deformation challenges and difficulty in positioning. This paper proposes an ultrafast laser processing system for trimming complex ceramic structural parts. Opto-electromechanical cooperative control software is developed to control the laser processing system. The trimming problem of the ceramic cores used in aero engines is studied. The regional registration method is introduced based on the iterative closest point algorithm to register the path extracted from the computer-aided design model with the deformed ceramic core. A zonal and layering processing method for three-dimensional contours on complex surfaces is proposed to generate the working data of high-speed scanning galvanometer and the computer numerical control machine tool, respectively. The results show that the laser system and the method proposed in this paper are suitable for trimming complex non-datum parts such as ceramic cores. Compared with the results of manual trimming, the method proposed in this paper has higher accuracy, efficiency, and yield. The method mentioned above has been used in practical application with satisfactory results.

关键词: ceramic parts trimming     computer-aided laser manufacturing     3D vision     reconfigurable laser processing system    

Hybrid forming mechanism of patternless casting and laser cladding

Zhongde SHAN, Fuzhen SUN, Yang LIU

《机械工程前沿(英文)》 2019年 第14卷 第4期   页码 393-401 doi: 10.1007/s11465-019-0550-1

摘要: In accordance with the requirement of manufacturing dies quickly and economically, a hybrid forming method of stamping dies for automobile panels is proposed. The method combines digital patternless casting and high-power laser cladding. An experimental study is conducted on the hybrid forming process and its trial production and application in the manufacturing of stamping dies for typical panels. Results prove that the laser cladding layer exceeds HRC60 (Rockwell hardness) and thus meets the production efficiency requirement of automobile dies. The rate of defects is well controlled. Compared with traditional technology, this technology has remarkable advantages and advancement.

关键词: patternless casting     laser cladding     hybrid forming     rapid tooling    

我国激光技术医疗应用和产业发展战略研究

邱海霞,李步洪,马辉,魏勋斌,杨思华,李阳,谭一舟,陈德福,赵洪友,曾晶,顾瑛

《中国工程科学》 2020年 第22卷 第3期   页码 14-20 doi: 10.15302/J-SSCAE-2020.03.003

摘要:

激光诊疗技术在生命健康领域得到了越来越多的成功应用,成为现代医学精准诊疗的重要组成部分。本文针对激光技术医疗应用主题,重点从实际应用和产业链的视角出发,梳理我国激光技术在临床治疗、诊断和产业化方面的发展现状、前景趋势和存在问题。研究发现,尽管我国在激光治疗技术、医用激光诊疗设备及其产业发展方面均取得了长足进步,但整体而言,激光医疗技术的临床应用仍处于跟踪起步和同步发展阶段,原创性应用研究有所缺乏,医用激光设备的研发和产业化处于中低端,用于精准诊疗的超快激光器等关键技术和高端设备仍未打破国外垄断。研究建议,设立我国激光医学二级学科,加强国家级激光医疗科技创新平台建设,针对性开展激光医疗器械法规建设,出台相关政策扶持激光医疗器械产业发展,组织攻关医用激光诊疗设备关键核心技术。

关键词: 激光技术     医疗     激光诊断     激光治疗     激光产业    

标题 作者 时间 类型 操作

Microcystin-LR biodegradation by

Chengbin XIAO, Hai YAN, Junfeng WANG, Wei WEI, Jun NING, Gang PAN

期刊论文

Oxidation and biotoxicity assessment of microcystin-LR using different AOPs based on UV, O

Siyi Lu, Naiyu Wang, Can Wang

期刊论文

Comparison of different algicides on growth of

Naiyu Wang, Kai Wang, Can Wang

期刊论文

Microcystin-LR detection based on indirect competitive enzyme-linked immunosorbent assay

SHENG Jianwu, HE Miao, YU Shaoqing, SHI Hanchang, QIAN Yi

期刊论文

工程网络计划的LR型模糊系数线性规划方法

高朋,冯俊文

期刊论文

激光去除与连接技术发展研究

陈俐,巩水利,何恩光

期刊论文

Temperature field simulation of laser homogenizing equipment

Juanjuan WANG, Yunshan WANG, Fudong ZHU

期刊论文

我国激光技术与应用2035 发展战略研究

“我国激光技术与应用2035 发展战略研究”项目综合组

期刊论文

Processing parameter optimization of fiber laser beam welding using an ensemble of metamodels and MOABC

期刊论文

Development of lunar regolith composite and structure via laser-assisted sintering

期刊论文

激光表面改性技术发展研究

张群莉,王梁,梅雪松,姚建华

期刊论文

Advances in polishing of internal structures on parts made by laser-based powder bed fusion

期刊论文

zone-layered trimming method for ceramic core of aero-engine blade based on an advanced reconfigurable laser

期刊论文

Hybrid forming mechanism of patternless casting and laser cladding

Zhongde SHAN, Fuzhen SUN, Yang LIU

期刊论文

我国激光技术医疗应用和产业发展战略研究

邱海霞,李步洪,马辉,魏勋斌,杨思华,李阳,谭一舟,陈德福,赵洪友,曾晶,顾瑛

期刊论文